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25 June 2017
 

Mohammad Saeid Hejazi


Title:     Professor of  Pharmaceutical Biotechnology
            MSc, PharmD, PhD 
Dept:    Pharmaceutical Biotechnology
Tel:       +98(41) 33372256
Fax:      +98(41) 33344798
E-mail:  msaeidhejazi@yahoo.com; saeidhejazi@tbzmed.ac.ir



EDUCATION AND QUALIFICATION

1999-2002: PhD in Molecular Medicine, with a thesis entitled “Cytoplasmic Fusagene Vectors” under the supervision of Prof. Farzin Farzaneh and Dr Joop Gäken. In this study a novel approach was developed to discover new and/or tissue specific cytoplasmic and possibly nuclear proteases. Proteases play a key role in cell physiology. They are involved in protein maturation, activation and processing. Therefore, they can be employed in processing of therapeutic proteins or other applications. For instance, recently furin endoprotease has been exploited to express secretary (e.g. IL-2, IL-4) and transmembrane molecules (e.g. B7.1) from a single cistron (i.e. a single expression vector). Therefore, identifying the tissue specific endoproteases, which cleave the specific sequences, will help to better understand cell biology and use them in different protocols. The developed system in this study provides an easy, reproducible and reliable tool to identify novel cytoplasmic endoprotease. Then the feasibility of the developed system was tested in various cell lines such as THP-1, HeLa, Saos-2 and PA1 cells.This system can be used to develop a new strategy to express multiple gene products inside the cytoplasm, using a single expression cassette. In this method multiple proteins are expressed as a single fusion protein, which is cleaved post synthetically into its constitutive proteins by cytoplasmic endoproteases. This strategy can be used as a general method in gene therapy to express multiple proteins inside the cytoplasm simultaneously, without the disadvantages associated with other protocols (e.g. using IRES and multiple promoters).

1998-1999: MSc in Immunology. Conducted research and presented a thesis entitled “Development of Tumour Cell Vaccination for Acute Myeloid Leukaemia (a murine model)” in AML immunogene therapy field under the supervision of Prof. Farzin Farzaneh. In this study IL-2/B7.1 and GFP encoding viruses were produced. Then IL-2 and B7.1 were expressed in AML cells and measured as secretory and transmembrane proteins by FACS and ELISA, respectively. The infected cells were irradiated and in mixed lymphocyte reaction –MLR- their ability to stimulate T cells proliferation was examined.

1984-1990: Pharm.D; Doctorate of pharmacy from Faculty of Pharmacy at Tabriz University of Medical Sciences. Conducted research and presented a thesis entitled “Formulation of nalidixic acid suspension” under the supervision of Prof. Barzegar Jalali. In this study different controlled flocculation protocols including chemical bridge, polymeric bridge, zeta potential reduction and liquid bridge methods were used to make stable suspensions. In addition, non-newtonian carriers were used to make stable suspensions.



EMPLOYMENT

1990-present:
  • Full-time junior lecturer at Faculty of Pharmacy, Tabriz University of Medical Sciences. Awarded a scholarship in order to study PhD from 1999-2002.
  • Full-time assistant-professor at Pharmaceutical Biotechnology Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, 2003-2008.
  • Full-time associate-professor at Pharmaceutical Biotechnology Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, 2008-2012.
  • Full-time professor at Pharmaceutical Biotechnology Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, February 2012- present.
  • Head and professor of Molecular Medicine Department, School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences.
  • Affiliated professor at Medical Biotechnology Department, School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences.
  • Affiliated professor at Medical Nanotechnology Department, School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences.           


ESTABLISHMENTS

  • Department of Pharmaceutical Biotechnology in Faculty of Pharmacy, Tabriz University of Medical Sciences. 2003.
  • Department of Molecular Medicine in Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences. 2008.
  • Cell Culture laboratory in Faculty of Pharmacy, Tabriz University of Medical Sciences. 2004.
  • Pharmaceutical Biotechnology laboratory in Faculty of Pharmacy, Tabriz University of Medical Sciences. 2004
  • Molecular Biology laboratory in Drug Applied Research Center, Tabriz University of Medical Sciences. 2003.
  • Molecular Medicine PhD course in Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences. 2007.
  • Pharmaceutical Biotechnology PhD course in Faculty of Pharmacy, Tabriz University of Medical Sciences. 2009.
  • Collaboration in nationwide establishment of Pharmaceutical Nanotechnology PhD course (in Iran).
  • Collaboration in establishment of Pharmaceutical Biomaterials PhD course in Faculty of Pharmacy, Tabriz University of Medical Sciences.
  • Collaboration in establishment of Medical Biotechnology PhD course in Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences.   
     

CURRENT POSITIONS

  • Head of Pharmaceutical Biotechnology Department at Faculty of Pharmacy, Tabriz University of Medical Sciences since 2004.
  • Head of Molecular Medicine Department at Faculty of Advanced Biomedical Sciences, Tabriz
    University of Medical Sciences since 2011. 
  • Member of Medical Biotechnology and Molecular Medicine national board since 2007.
  • Member of Pharmaceutical Biotechnology national board since 2010. 


TEACHING EXPERIENCE

1990-1998:            Biological Products,
                              
Physico-chemical Control of Pharmaceutical Products.

2002-present:         Cell Culture, 
                              
Molecular Biology, 
                              
Genetic Engineering and Biotechnology, 
                              
Biological Products.


BACTERIAL TAXONOMIC STUDIES
 
 

Discovery of a novel bacterial genus called Tabrizicola aquatica. The corresponding article is published in Antonie van Leeuwenhoek journal as “Tabrizicola aquatica gen. nov. sp. nov., a novel alphaproteobacterium isolated from Qurugöl Lake nearby Tabriz city, Iran”.

Discovery of a novel bacterial species called Alishewanella tabrizica. The corresponding article is published in International Journal of Systematic and Evolutionary Microbiology as “Alishewanella tabrizica sp. nov., isolated from Qurugöl Lake in mountainous region of Azerbaijan at northwest of Iran”.

 

Publications

BOOK:

Hejazi M S. Biological products in prevention, diagnosis and treatment of diseases. 1999, Nashre Tabib, Tehran. ISBN: 964-456-129-5

ARTICLES: 

  1. Sharifi S, Hejazi MS, Barar J, Samadi N. Role of Bcl-2/Bax ratio, caspase-8 and 9 in resistance of breast cancer cells to paclitaxel. Asian Pacific Journal of Cancer Prevention 2014;15(20): 8617-22.
  2. Hamidi M, Zainul Abdin M, Nazemyieh H, Hejazi MA, Hejazi MS. Optimization of total carotenoid production by Halorubrum sp. TBZ126 using response surface methodology. Journal of Microbial & Biochemical Technology 2014; 6(5): 286-294.
  3. Hamidi M, Naziri D, Hassanzadeh S, Maleki Zanjani B, Abdin MZ, Hejazi MA, Nazemyieh H, Hejazi MS. Carotenoid profile of an extreme haloalkaliphile archaeon, Halorubrum sp. TBZ112 isolated from Urmia Lake. BioImpacts. Accepted.
  4. Hajizadeh N, Sefidi Heris Y, Zununi Vahed S, Vallipour J, Hejazi MA, Golabi SM, Asadpour-Zeynali K, Hejazi MS. Biodegradation of para-amino acetanilide by Halomonas sp. TBZ3, a slightly halophilic bacterium isolated from Urmia Lake in Iran. Jundishapur Journal of Microbiology. Accepted.
  5. Hejazi MS, Majidi MR, Gholizadeh S, Hamidi-Asl E, Turner APF, Golabi SM. Effect of Electrophoresis on the Efficiency of Nano-TiO2 Modified Silica Sol-gel Electrode. Journal of Nanoscience and Nanotechnology 2015; 15: 3405–3410.
  6. Mehdizadeh Aghdam E, Barzegar A, Hejazi MS. Evolutionary origin and conserved structural building blocks of riboswitches and ribosomal RNAs: riboswitches as probable target sites for aminoglycosides interaction. Advanced Pharmaceutical Bulletin 2014; 4(3): 225-235.
  7. Naziri D, Hamidi M, Hassanzadeh S, Tarhriz V, Maleki Zanjani B, Nazemyieh H, Hejazi, MA, Hejazi MS. Analysis of carotenoid production by Halorubrum sp. TBZ126; an extremely halophilic archeon from Urmia Lake. Advanced Pharmaceutical Bulletin 2014; 4(1): 61-67.
  8. Hamidi-Asl E, Raoof JB, Ojani R, Hejazi MS. Indigo carmine as new label in PNA biosensor for detection of short sequence of p53 tumor suppressor gene. Electroanalysis 2013; 25(9): 2075-2083.
  9. Ghotaslou R, Milani M, Akhi MT, Hejazi MS, Nahaei MR, Hasani A, Sharifi Y. Relationship Between Drug Resistance and cagA Gene in Helicobacter pylori. Jundishapur Journal of Microbiology 2013; 6(10): e8480, DOI: 10.5812/jjm.8480.
  10. Tarhriz V, Thiel V, Nematzadeh GA, Hejazi MA, Imhoff JF, Hejazi MS. Tabrizicola aquatica gen. nov. sp. nov., a novel alphaproteobacterium isolated from Qurugöl Lake nearby Tabriz city, Iran. Antonie van Leeuwenhoek 2013; 104:1205–1215.
  11. Hamidi-Asl E, Raoof JB, Ojani R, Golabi SM, Hejazi MS. A new peptide nucleotide acid biosensor for electrochemical detection of single nucleotide polymorphism in duplex DNA via triplex structure formation. Journal of the Iranian Chemical Society 2013; 10: 1075-1083.
  12. Ahour F, Pournaghi-Azar MH, Alipour E, Hejazi MS. Detection and discrimination of recombinant plasmid encoding hepatitis C virus Core/E1 gene based on PNA and double-stranded DNA hybridization. Biosensors and Bioelectronics 2013; 45(1): 287-291.
  13. Mahmoudi Azar L, Mehdizadeh Aghdam E, Karimi F, Haghshenas B, Barzegari A, Yaghmaei P, Hejazi MS. Influence of foreign DNA introduction and periplasmic expression of recombinant human interleukin-2 on hydrogen peroxide quantity and catalase activity in Escherichia coli. Advanced Pharmaceutical Bulletin 2013; 3(2): 395-402.  
  14. Mesgari Shadi A, Yaghmaei S, Vafaei F, Khataee AR, Hejazi MS. Degradation of benzene, toluene, and xylene (BTX) from aqueous solution by isolated bacteria from contaminated sites. Research on Chemical Intermediates. Published online April 2013.
  15. Arami S, Pournaghi-Azar MH, Alipour E, Hejazi MS. Voltammetric detection of uridin diphosphate glucuronosyl transferase 1A9 (UGT1A9) gene corresponding oligonucleotide covering promoter region from -268 to -280 including (A/T) polymorphism at position -275 and optimization of the detection factors. Journal of the Iranian Chemical Society 2013; 10(3): 399-406.
  16. Ghotaslou R, Yeganeh Sefidan F, Akhi MT, Soroush MH, Hejazi MS. Detection of legionella contamination in Tabriz hospitals by PCR assay. Advanced Pharmaceutical Bulletin 2013; 3(1): 131-134.  
  17. Ghotaslou R, Milani M, Akhi MT, Nahaei MR, Hasani A, Hejazi MS, Meshkini M. Diversity of Helicobacter Pylori cagA and vacA genes and its relationship with clinical outcomes in Azerbaijan, Iran. Advanced Pharmaceutical Bulletin 2013; 3(1): 57-62.
  18. Mehdizadeh Aghdam E, Mahmoodi Azar L, Barzegari A, Karimi F, Mesbahfar M, Samadi N, Hejazi MS. Effect of periplasmic expression of recombinant mouse interleukin-4 on hydrogen peroxide concentration and catalase activity in Escherichia coli. Gene 2012; 511(2): 455-460.
  19. Dalir Abdolahinia E, Nazemiyeh H, Hejazi MS. Genotype comparison of Azarbaijan native citronellol producing Thyme with Thymus pubescens. Advances in Bioresearch 2012; 3(2): 36 – 39.
  20. Ahour F, Pournaghi-Azar M H, Hejazi MS. An electrochemical approach for direct detection and discrimination of  fully match and single base mismatch double-stranded oligonucleotides corresponding to universal region of hepatitis C virus. Analytical Methods 2012; 4(4): 967-972.
  21. Tarhriz V, Nematzadeh G A, Zununi Vahed S, Hejazi M A, Hejazi MS. Alishewanella tabrizica sp. nov., isolated  from Qurugöl Lake in mountainous region of Azerbaijan at northwest of Iran. International Journal of Systematic and Evolutionary Microbiology 2012; 62(8): 1986-1991.
  22. Alvari A, Ohadi Rafsanjani M S, Ahmed F J, Hejazi MS, Abdin M Z. Rapid RP-HPLC technique for the  determination of phyllanthin as bulk and its quantification in Phyllanthus amarus extract. International Journal of  Phytomedicine 2011; 3 (1): 115-119.
  23. Dalir Abdolahinia E, Sokhandan Bashir N, Hagnazari A, Nazemiyeh H, Hejazi MS. A Comparative Phenotypic  and ITS Based Genotypic Study in Thyme species (Thymus L. Lamiaceae). VEGETOS 2011; 24 (2): 102-113.
  24. Tarhriz V, Nematzadeh G A, Mohammadzadeh F, Rahimi R, Hejazi MS. Isolation and characterization of some aquatic bacteria from Qurugöl Lake in  Azerbaijan under aerobic conditions. Advances in Environmental Biology 2011; 5 (10): 3173-3178.  
  25. Ilkhani H, Ganjali M R, Arvand M, Hejazi MS, Azimi F, Norouzi P. Electrochemical spectroscopic investigations on the interaction of an ytterbium complex with DNA and their analytical applications such as biosensor.  International Journal of Biological Macromolecules 2011; 49(5): 1117-1123. 
  26. Zununi Vahed S, Forouhandeh H, Hassanzadeh S, Klenk H-P, Hejazi M A, Hejazi MS. Isolation and characterization of halophilic bacteria from Urmia Lake in Iran. Microbiology (Moscow) 2011; 80 (6): 826-833.
  27. Raoof J B, Ojani R, Golabi S M, Hamidi-Asl E, Hejazi MS. Preparation of an electrochemical PNA biosensor for  detection of target DNA sequence and single nucleotide mutation on p53 tumor suppressor gene corresponding  oligonucleotide. Sensors and Actuators B: Chemical 2011; 157: 195-201.
  28. Sehatnia B, Golabi F, Pournaghi-Azar M H, Sabzi R E, Hejazi MS. Modeling of DNA hybridization detection using methylene blue as an electroactive label. Journal of the Iranian Chemical Society 2011;  8 (1): 115-122.
  29. Majidi M R, Gholizadeh S, Hejazi MS. Immobilization of lactate as an electroactive indicator on pencil graphite  electrode for the development of a new electrochemical biosensor for the detection o lactate dehydrogenase.  Journal of the Iranian Chemical Society 2011; 8 (1): 59-67.
  30. Nasirizadeh N, Zare HR, Pournaghi-Azar MH, Hejazi MS. Introduction of hematoxylin as an electroactive label for DNA biosensors and its employment in detection of target DNA sequence and single-base mismatch in human papilloma virus corresponding to oligonucleotide. Biosensors and Bioelectronics 2011; 26 (5): 2638-2644.
  31. Shayegh J, Parvizi M, Hejazi MS. Diversity of caprine and ovine Pasteurella multocida isolates based on 16S  rRNA gene sequencing. Iranian Journal of Veterinary Research, Shiraz University 2010; 11 (4): 373-378.
  32. Alipour E, Pournaghi-Azar MH, Parvizi M, Golabi SM, Hejazi MS. Electrochemical detection and discrimination of single copy gene target DNA in non-amplified genomic DNA. Electrochimica Acta 2011; 56 (5): 1925-1931.
  33. Shayegh J, Atashpaz S, Zahraei Salehi T, Hejazi MS. Potential of Pasteurella multocida isolated from healthy  and diseased cattle and buffaloes in induction of disease. Bulletin of Veterinary Institute in Pulawy 2010; 54 (3): 299-304.
  34. Hejazi MS, Pournaghi-Azar MH, Alipour E, Dalir Abdolahinia E, Arami S, Navvaha H. Development of a Novel Electrochemical Biosensor for Detection and Discrimination of DNA Sequence and Single Base Mutation in dsDNA Samples Based on PNA-dsDNA Hybridization – a new Platform Technology. Electroanalysis 2011; 23 (2): 503-511.
  35. Gol Mohammad Zade Khiaban N, Haddad Irani-Nejad K, Hejazi MS, Mohammadi S A, Sokhandan N. A  geometric morphometric study on geographical Iranian populations of the pod borer, Helicoverpa armigera  (Lepidoptera: Noctuidae). Journal of Entomological Society of Iran 2010; 29 (2): 13-24.
  36. Pournaghi-Azar MH, Ahour F, Hejazi MS. Direct detection and discrimination of double-stranded oligonucleotide corresponding to hepatitis  C virus genotype 3a using an electrochemical DNA biosensor based on peptide nucleic acid and double-stranded DNA hybridization. Analytical and Bioanalytical Chemistry 2010; 397 (8):3581–3587.
  37. Gol Mohammad Zade Khiaban N, Hejazi MS, Haddad Irani-Nejad K, Mohammadi S A, Khaghaninia S.Genetic variability of geographical populations of  the bollworm, Helicoverpa armigera hübner (Lepidoptera: Noctuidae), in west and northwest of Iran. Munis Entomology & Zoology 2010; 5 (2): 670-676.
  38. Khani S, Sohani MM, Mahna N, Barar J, Hejazi MS, Nazemieh H, Atashpaz S, Dadpour M R, Omidi Y. Cloning of taxadiene synthase gene into Arabidopsis thaliana (ecotype Columbia-0). African Journal of  Biotechnology 2010; 9 (12): 1734-17400.
  39. Hejazi MA, Barzegari A, Hosseinzadeh Gharajeh N, Hejazi MS. Introduction of a novel 18S rDNA gene arrangement along with distinct ITS region in the saline water microalgae Dunaliella. Saline Systems 2010; 6 (1), art. no.  4.
  40. Forouhandeh H, Zununi Vahed S, Hejazi MS, Nahaei M R, Akbari Dibavar M. Isolation and phenotypic   characterization of Lactobacillus species from various dairy products. Current Research in Bacteriology 2010; 3(2): 84-88.
  41. Barzegari A, Hejazi MA, Hosseinzadeh N, Eslami S, Mehdizadeh Aghdam E, Hejazi MS. Dunaliella as an  attractive candidate for molecular farming. Molecular Biology Reports 2010; 37 (7): 3427-3430.
  42. Raoof JB, Hejazi MS. Ojani R, Hamidi Asl E. A comparative study of carbon nanotube paste electrode for development of indicator-free DNA biosensor using DPV and EIS: human interleukin-2 oloigonucleotide as a model. International Journal of Electrochemical Science 2009; 4 (10): 1436-1451.
  43. Kalbasi A, Faramarzi M A, Hejazi MS, Jahandar H, Amini M, Jalali SM. 14α-hydroxylation of androst-4-en-3,  17-dione by the whole cells of cyanobacterium Nostoc piscinale. Biotechnology 2009; 8 (3): 370-374.
  44. Aminnejad M, Babai-Ahari A, Javan-Nikkhah M, Hejazi MS. Molecular and pathogenic variation within Iranian Pyrenophora graminea population: more polymorphism in IGS region. American-Eurasian Journal of Agricultural  & Environmental Sciences 2009; 6 (6): 697-704.
  45. Ebrahimi A A, Noshad H, Sadreddini Sh, Hejazi MS, Mohammadzadeh Sadigh Y, Eshraghi Y, Ghojazadeh M. Serum levels of TNF-α, TNF-αRI, TNF-αRII and IL-12 in treated rheumatoid arthritis patients. Iranian Journal of  Immunology 2009; 6 (3): 147-153.
  46. Hejazi MS, Pournaghi-Azar MH, Ahour F. Electrochemical detection of short sequences of hepatitis C 3a virus   using a peptide nucleic acid-assembled gold electrode. Analytical Biochemistry 2010; 399 (1): 118-124.
  47. Khiaban N G M Z, Haddad Irani Nejad K, Hejazi MS, Mohammadi SA, Sokhandan N. A Geometric morphometric study on the host populations of the pod borer, Helicoverpa armigera (Hübner) (Lepidoptera:  Noctuidae) in some parts if Iran. Munis Entomology & Zoology 2010; 5 (1): 140-147.
  48. Atashpaz S, Shayegh J, Hejazi MS. Rapid virulence typing of Pasteurella multocida by multiplex PCR. Research in Veterinary Science 2009; 87 (3): 355-357.
  49. Hejazi MS, Karimi F, Mehdizadeh Aghdam E, Barzegari A, Farshdosti Hagh M, Parvizi M, Mahmoodi Azar L, Hejazi MA. Cytoplasmic expression of recombinant interleukin-2 and interleukin-4 proteins results in hydrogen  peroxide accumulation and reduction in catalase activity in Escherichia coli. DARU 2009; 17 (2): 64-71.   
  50. Pournaghi-Azar MH, Ahour F, Hejazi MS. Differential pulse voltammetric detection of hepatitis C virus 1a  oligonucleotide chain by a label-free electrochemical DNA hybridization biosensor using consensus sequence of  hepatitis C virus 1a probe on the pencil graphite electrode. Electroanalysis 2009; 21 (16): 1822-1828.
  51. Hejazi MS, Raoof J B, Ojani R, Golabi S M, Hamidi Asl E. Brilliant cresyl blue as electroactive indicator in electrochemical DNA oligonucleotide sensors. Bioelectrochemistry 2010; 78 (2): 141-146.
  52. Sofalian O, Chaparzadeh N, Javanmard A, Hejazi MS. Study the genetic diversity of wheat landraces from  northwest of Iran based on ISSR molecular markers. International Journal of Agriculture & Biology 2008; 10 (4): 466-468.
  53. Chaparzadeh, N, Sofalian O, Javanmard A, Hejazi MS, Zarandi L. Study of glutenin subunits in some wheat  landraces from northwest of Iran by SDS-PAGE technique. International Journal of Agriculture & Biology 2008; 10 (1): 101-104.
  54. Sabzi RE, Sehatnia B, Pournaghi-Azar MH, Hejazi MS. Electrochemical detection of human papilloma virus (HPV) target DNA using MB on pencil graphite electrode. Journal of the Iranian Chemical Society 2008; 5 (3): 476-483.
  55. Shayegh J, Atashpaz S, Hejazi MS. Virulence genes profile and typing of ovine Pasteurella multocida. Asian  Journal of Animal and Veterinary Advances 2008; 3 (4): 206-213.
  56. Hejazi MS, Sioofy Khojine A B. Influence of E1-deleted recombinant adenoviruses on B7.1 and IL-2 expression in C1498 cells. Iranian Biomedical Journal 2007; 11 (3): 153-160.
  57. Hejazi MS, Ghotaslou R, Farshdoosty Hagh M, Mohammadzadeh Sadigh Y. Genotyping of hepatitis C virus in northwest of  Iran. Biotechnology 2007; 6 (3): 302-308.
  58. Sokhandan Bashir N, Nourinejhad Zarghani Sh, Hejazi MS. Diversity of Grapevine fanleaf virus isolates fro  Iran. Virus Research 2007; 128 (1-2): 144-148.
  59. Zereshki Nobar L, Azarbaijani R, Valizadeh M, Hejazi MS. Cloning and sequencing of ABC transporter ATP-binding protein encoding gene from Streptomyces minoensis.  Biotechnology 2008; 7 (2): 182-187.
  60. Hejazi MS, Kazemi Tabar K, Azarbaijani R, Zereshki Nobar L. Cloning and sequencing of partial segment of  cholesterol oxidase encoding gene from Streptomyces luridus. Annals of Microbiology 2007; 57 (2) 259-263.
  61. Pournaghi-Azar MH, Alipour E, Zununi S, Froohandeh H, Hejazi MS. Direct and rapid electrochemical  biosensing of the human interleukine-2 DNA in unpurified polymerase chain reaction (PCR)-amplified real samples. Biosensors and Bioelectronics 2008; 24 (4): 524-530.
  62. Hejazi MS, Pournaghi-Azar MH, Alipour E, Karimi F. Construction, electrochemically biosensing and discrimination of recombinant plasmid (pEThIL-2) on the basis of interleukine-2 DNA insert. Biosensors and Bioelectronics 2008; 23 (11): 1588-1594.
  63. Hejazi MS, Alipour E, Pournaghi-Azar MH. Immobilization and voltammetric detection of human interleukine-2 gene on the pencil graphite electrode. TALANTA 2007; 71 (4): 1734-1740.
  64. Pournaghi-Azar MH, Hejazi MS, Alipour E. Detection of human interleukine-2 gene using a label-free electrochemical DNA hybridization biosensor on the basis of a non-inosine substituted probe. Electroanalysis 2007; 19 (4): 466-472.
  65. Pournaghi-Azar MH, Hejazi MS, Alipour E. Developing an electrochemical deoxyribonucleic acid (DNA)  biosensor on the basis of human interleukine-2 gene using an electroactive label. Analytica Chimica Acta 2006; 570 (2): 144-150.
  66. Hejazi MS. Evaluation of plasmid pCCI in induction of resistance and sensitivity to zeocin and GCV in mammalian cells. Pharmaceutical Sciences Journal of Tabriz University of Medical Sciences 2005; 2: 21-32.
  67. Hejazi MS, Farzaneh F. Simultaneous expression of interleukin-2 and B7.1 using a recombinant adenovirus  vector with a single expression cassette containing IRES. Pharmaceutical Sciences Journal of Tabriz University of  Medical Sciences 2003; 1: 83-97.
  68. Hejazi MS, Gaken J, Farzaneh F. Employment of recombinant fusion proteins to develop a novel system for identification of cytoplasmic endoproteases. Medical Journal of Tabriz University of Medical Sciences & Health Services 2005; 27 (2): 29-36.
  69. Hejazi MS. Non-aqueous potentiometeric assay of diltiazem hydrochloride in tabletsPharmaceutical Sciences  Journal of Tabriz University of Medical Sciences 2000; 2: 97-102.
  70. Parvizi R, Hejazi MS, Jelvegari M, Hasanzadeh D, Garjani A, Babaei H, Azizian P. Formulation of cardioplegia  ampule and comparison of its in vitro characters and in vivo effects with commercial sample. Pharmaceutical  Sciences Journal of Tabriz University of Medical Sciences 2000; 2: 81-88.
  71. Hejazi MS, Barzegar Jalali M. Formulation and evaluation of some nalidixic acid suspensions. Pharmaceutical Sciences  Journal of Tabriz University of Medical Sciences 1995; 2: 60-66.

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SUPERVISED THESIS


1. Development of electrochemical DNA biosensor for detection of HCV gene based on differential pulse voltammetry. PhD thesis, 2011 (Supervisor).

2. Evaluation of dye compounds biodegradation by halophilic bacteria isolated from Urmia Lake and optimization of  the biodegradation conditions. MSc thesis, 2011 (Supervisor).

3. Evaluation of aromatic compounds biodegradation and ferric ion reduction by bacterial isolates TBZ9 and TBZ23  isolated from Qurugöl Pond and optimization of biodegradation conditions. MSc thesis, 2011 (Supervisor).

4. Investigation of electrochemical behavior of some of biological compounds and their application for development of  DNA, adernaline, noradernaline and acetaminophen biosensors using nanomaterial modified platforms. PhD thesis, 2011 (Consultant).

5. Identification of Urmia Lake shores halophilic bacteria by biochemical methods and polymerase chain reaction. MSc thesis, 2011 (Supervisor).

6. Design and construction of novel electrochemical biosensors for detection and determination of biological materials and biomarkers. PhD thesis, 2010 (Supervisor).

7. A geometric, morphometric and molecular study on populations of the pod borer, Helicoverpa armigera (Hübner) (Lep.: Noctuidae) in Northwest region and Golestan province of Iran. PhD thesis, 2010 (Supervisor).

8. Study of presence and biodiversity of Marinobacter sp. in Gurugöl Lake. MSc thesis, 2010 (Supervisor).

9. Expression of Iranian isolate of Grapevine fanleaf virus  movement protein gene in Escherichia coli. MSc thesis, 2010 (Consultant).

10. Effect of cytoplasmic recombinant proteins expression on hydrogen peroxide concentration and catalase activity in Escherichia coli. PharmD thesis, 2009 (Supervisor).

11. Diversity study of halophilic bacteria being in mud samples of Urmia Lake by molecular methods. MSc thesis, 2009 (Supervisor).

12. Expression of recombinant coat protein of Cucumber mosaic virus in Escherichia coli for antiserum preparation. MSc thesis, 2009 (Consultant).

13. Evaluation of periplasmic expression of recombinant human interleukin-2 protein influence on H2O2 concentration and catalase activity in Escherichia coli. MSc thesis, 2009 (Supervisor)

14. Development of a PNA biosensor for diagnosis of uridin diphosphate glucronosyltransferase 1A9 (UGT1A9) gene promoter region polymorphism at position -275. PharmD thesis, 2009 (Supervisor).

15. Study on the biotransformation of androst-4-en-3,17-dione by Nostoc sp. PharmD thesis, 2009 (Supervisor).

16. Development of electrochemical DNA biosensor on the basis of human interleukin-2 gene and its application for detection of recombinant plasmids. PhD thesis, 2008 (Supervisor).

17. Comparison between phenotypic and genotypic characteristics of Thymus spp. in Azarbaijan. MSc thesis, 2008 (Supervisor).

18. Detection of human Papilloma virus (HPV) in lung and oral squamous cell carcinomas by PCR in Northwest Iran. PhD thesis, 2008 (Supervisor).

19. Transformation of Arabidopsis thaliana with Taxadiene synthase gene as a first committed step in taxol biosynthesis pathway. MSc thesis, 2008 (Consultant).

20. Study of presence and genetic diversity of Halomondaceae bacteria in Urmia Lake. MSc thesis, 2008 (Supervisor).

21. Genotyping of Iranian ovine and bovine Pasteurella multocida isolates using multiplex PCR method based on capsular genes. PhD thesis, 2008 (Supervisor).

22. Genotype characterization of Lactobacillus isolates from the Northwest Iran. MSc thesis, 2008 (Supervisor).

23. Development of an electrochemical DNA biosensor for detection of Human Papilloma virus (HPV) target DNA. MSc thesis, 2008 (Supervisor).

24. Biodiversity study of some wheat landraces in Northwest Iran by storage proteins and molecular markers. MSc thesis, 2008 (Consultant).

25. Evaluation of interleukin-4 gene promoter region genetic polymorphism in patient with relapsing visceral leishmaniasis. DVM thesis, 2007 (Supervisor).

26. Development of a new method for sex determination of bovine using ZFY and ZFX genes specific primers based on PCR. MSc thesis, 2006 (Supervisor).

27. Employment of pET22b vector for cloning and expression of interleukin-2 cDNA in the periplasm of  E. coli. MSc thesis, 2006 (Supervisor).

28. Evaluation of possibility for cloning and sequencing of tannase encoding gene from Bacillus cereus. PharmD thesis, 2006 (Supervisor).

29. Subcloning of interleukin-4 cDNA and its periplasmic expression in E. coli using a prokaryotic expression vector. MSc thesis, 2006 (Supervisor).

30. Cloning and sequencing of cholesterol oxidase encoding gene from Streptomyces luridus. MSc thesis, 2006 (Supervisor).

31. Cloning and sequencing of partial fragment of cholesterol oxidase encoding gene from Streptomyces narbonensis. MSc thesis, 2006 (Supervisor).

32. Evaluation of genetic diversity and pathogenicity of Pyrenophora graminea isolates in East Azarbaijan province,Iran. MSc thesis, 2006 (Supervisor).

33. Construction of a prokaryotic expression vector for expression of interleukin-4 in E. Coli. PharmD thesis, 2006 (Supervisor).

34. Cytoplasmic endoproteases for cleavage of fusion proteins as therapeutic targets. PhD thesis, 2006 (Supervisor).

35. Expression of recombinant coat protein of grapevine fanleaf virus for antiserum preparation. MSc thesis, 2006 (Consultant).

36. Evaluation of interleukin-10 polymorphism in visceral leishmaniasis patients and their close relevants (comparison of IL-10 promoter region polymorphism in drug resistant patients and their relatives). PharmD thesis, 2005  (Supervisor).

37. Construction of pB7G4VP plasmid and evaluation of B7.1/GAL4-VP16 chimeric recombinant protein expression in cytoplasmic membrane of eukaryotic cells. PharmD thesis, 2005 (Consultant).

38. Cloning and expression of human interleukin-2 in eukaryotic cells. PharmD thesis, 2005 (Supervisor).

39. Sub-cloning of interleukin-2 encoding cDNA in prokaryotic expression vector and evaluation of its expression in E. Coli. MSc thesis, 2004 (Supervisor).

40. Formulation of cardioplegia pareteral products and comparison of them with foreigner samples. PharmD thesis, 1999 (Supervisor).

41. Formulation of captopril matrix tablets. PharmD thesis, 1999 (Consultant).

42. Evaluation of chemical stability of diltiazem hydrochloride solutions. PharmD thesis, 1999 (Supervisor).

43. Evaluation of diltiazem hydrochloride release rate from matrices. PharmD thesis, 1999 (Supervisor).

44. Comparison of analytical methods of H2 blocker products (cimetidine, ranitidine, famotidine). PharmD thesis,1999 (Supervisor)

45. Physical stability and release studies of nalidixic acid suspensions. PharmD thesis, 1995 (Supervisor).


 

 

 

Abstracts (some of):

 

 

 

Raoof JB, Hejazi M S, Ojani R, Golabi S M, Hamidi Asla E. A DNA biosensor based on peptide nucleotide acids using 1,1'- dianthrimid as a new electroactive indicator. 5th Electrochemistry Seminar of Iran. May 2009, Tehran, Iran.

Hejazi M S. DNA biosensors and their applications. First Regional Symposium on Bioelectrochemistry. October 2008. Institute of Biochemistry and Biophysics, University of Tehran, Iran. (Invited Speaker).

Hejazi M S. Reactive oxygen specious and their role in recombinant protein expression in Escherichia coli. 11th Iranian Pharmaceutical Sciences Conference, August 2008, Kerman, Iran. (Invited Speaker).

Zununi Vahed S, Fruhande H, Chaparzade N, Hejazi M A, Nahaei M R, Sofalian O, Dibavar M A, Hejazi M S. Isolation and identification a novel Iranian native bacterium belonging to Marinobacter genus. The 5th National Biotechnology Congress of Iran. November 2007, Tehran, Iran.

Fruhande H, Zununi Vahed S, Nahaei M R, Akbari Dibavar M, Hejazi M S. Genotypic and phenotypic study of Lactobacillus species isolated from local dairy products of Tabriz. The 5th National Biotechnology Congress of Iran. November 2007, Tehran, Iran.

Atashpaz S, Shaeygh J, Hejazi M S. Design of multiplex PCR for Virulence Typing of Pasteurella multocida Strains Isolated from Different Host with Various Disease States. The 5th National Biotechnology Congress of Iran. November 2007, Tehran, Iran.

Hejazi M S, Parvizi M. Single Nucleotide Polymorphism at Position -590(C/A) in Patients with Relapsing Visceral Leishmaniasis Might attribute to the Drug Resistance. The 9th Iranian Congress of Biochemistry & the 2nd International Congress of Biochemistry and Molecular Biology. October-November 2007, Shiraz, Iran.

Hejazi M S, Pournaghi-Azar M H, Alipour E. Development of an electrochemical biosensor for detection of PCR product of human interleukin-2 encoding DNA. The 9th Iranian Congress of Biochemistry & the 2nd International Congress of Biochemistry and Molecular Biology. October-November 2007, Shiraz, Iran.

Arami S, Alipour E, Arami S, Hejazi M S, Pournaghi-Azar M H. Electrochemical detection of UGT1A9 -275 (T/A) single nucleotide polymorphism using PNA-gold biosensor. The 9th Iranian Congress of Biochemistry & the 2nd International Congress of Biochemistry and Molecular Biology. October-November 2007, Shiraz, Iran.

Pournaghi-Azar M H, Hejazi M S, Alipour E. Developing of a label-free electrochemical DNA hybridization biosensor for detection of human interleukin-2 gene as recombinant plasmid containing human iterleukin-2 encoding DNA. 7th Biennial Electrochemistry Seminar of Iran. August 2007, Urmia, Iran.

Sehatnia B, Pournaghi-Azar M H, Hejazi M S, Alipour E, Sabzi R. Electrochemical detection of human papilloma virus (HPV) using MB on pencil graphite electrode. 7th Biennial Electrochemistry Seminar of Iran. August 2007, Urmia, Iran.

Atashpaz S, Hejazi M S, Ghotaslu R, Farshdoosti M, Parvizy M. Genotyping of hepatitis C virus in northwest of Iran. 14 th International Student Congress of Medical Sciences (ISCOMS). June 2007, Groningen,The Netherlands.

Parvizi M, Hejazi M S. Presence of single nucleotide polymorphism at position -34 (C/T) in patients with relapsing visceral leishmaniosis. International Medical & Health Congress (IMHC) 2007. May 2997, Kelantan,Malaysia.

Shayegh J, Hejazi M S, Tabatabayi A M, Sharaf J D. Phenotyping of Pasteurella multocida isolates from cattle, sheep and buffalo in Iran. First Iranian Congress of Clinical Microbiology. May 2007, Shiraz, Iran.

Pournaghi-Azar M H, Hejazi M S, Alipour E. Detection of human interleukin-2 gene using label-free electrochemical DNA hybridization biosensor. 15th Iranian Seminar of Analytical Chemistry. February-March 2007, Shiraz, Iran.

Pournaghi-Azar M H, Hejazi M S, Alipour E. Developing an electrochemical DNA biosensor on the basis of sense strand of human interleukin-2 gene using a pencil lead electrode and methylene blue as an electroactive label. International Composites Conference: Developments in Composites: Advanced, Infrastructural, Natural & Nano-composites. July 2006, Sydney, Australia.

Hejazi M S, Kazemi tabar K, Valizadeh M. Azarbaijani R. Cloning and sequencing of oxido-reductase domain of cholesterol oxidase gene from Streptomyces luridus. 10th Iranian Pharmaceutical Sciences Conference. August 2006, Tehran, Iran.

Hejazi M S, Alipour E, Pournaghi Azar M H. Development of a label-free biosensor for DNA detection using an anti-sense strand oligonucleotide related to human interleukine-2 gene. 10th Iranian Pharmaceutical Sciences Conference. August 2006, Tehran, Iran.

Hejazi M S, Barzgari A. Cloning and inducible periplasmic expression of IL-4 in E. coli using T7 promoter. 10th Iranian Pharmaceutical Sciences Conference, August 2006, Tehran, Iran.

Hejazi M S, Haghshenas B. Cloning and periplasmic expression of interleukin- 2 in E. coli under the control of T7 promoter. 10th Iranian Pharmaceutical Sciences Conference, August 2006, Tehran, Iran.

Hejazi M S, Mesbahfar M. Cloning and inducible expression of interleukin-4 inside the cytoplasm of Escherichia coli. 10th Iranian Pharmaceutical Sciences Conference, August 2006, Tehran, Iran.

Hejazi M S, Moharrami M R, Farshdosty hagh M. Cloning and sequencing of a partial segment of dihydrodipicolinate synthetase from Bacillus Cereus ATCC:11778. 10th Iranian Pharmaceutical Sciences Conference, August 2006, Tehran, Iran.

Hejazi MS, Parvizy M. A novel single nucleotide polymorphism at position -387 in human interleukin-12 promoter region. 10th Iranian Pharmaceutical Sciences Conference, August 2006, Tehran, Iran.

Hejazi M S, Zereshki Nobar L, Babaeian N, Valizadeh M, Kazemi A. Cloning and partial sequencing of cholesterol oxidase encoding gene from Streptomyces narbonensis. 10th Iranian Pharmaceutical Sciences Conference, August 2006, Tehran, Iran.

Hejazi M S, Zereshki Noubar L, Azarbaijani R, Kazemi A. Partial cloning and sequencing of amino acid permease encoding gene from Streptomyces minoensis. 4th Iranian National Biotechnology Congress, August 2005, Kerman, Iran.

Hejazi M S, Azarbaijani R, Zereshki Noubar L, Mohammadzadeh S. Partial cloning and sequencing of beta prime peptide chain encoding gene from Streptomyces rimosus. 4th Iranian National Biotechnology Congress, August 2005, Kerman, Iran.

Hejazi M S, Kazemitabar S K, Valizadeh M, Azarbaijani R, Zereshki Noubar L, Mohammadzadeh S. Partial cloning and sequencing of cholesterol oxidase encoding gene from Streptomyces minoensis. 4th Iranian National Biotechnology Congress, August 2005, Kerman, Iran.

Hejazi M S, Babaloo Z, Rafati S, Shafag A, Ghanbarian H. A novel single nucleotide polymorphism in IL-10 promoter region at position -47 in relatives of patients with visceral leishmaniasis. 13th Iranian Congress on Infectious Diseases and Thropical Medicine, December 2004, Tehran, Iran.

Hejazi M S, Karimi F, Ghanbarian H, Mohammadzadeh Y, Baghban B, Valizadeh M. Cloning and expression of human interleukin-2 in E.coli. 9th Iranian Seminar of Pharmaceutical Sciences, August 2004, Tabriz, Iran.

Kazemi A, Robson G D, Denning D, Hejazi M S, Asgharzadeh M. Cloning and sequencing of partial fluconazole resistance gene of Aspergillus fumigatus. 9th Iranian Seminar of Pharmaceutical Sciences, August 2004, Tabriz, Iran.

Hejazi M S, Gaken J, Lotfipour F, Ghanbarian H, Farzaneh F. Development of a new biological system for identification of novel and tissue specific cytoplasmic endoproteases. 3rd Iranian National Biotechnology Congress, September 2003, Mashhad, Iran.

Hejazi M S, Gaken J, Farzaneh F. A new strategy for development of cytoplasmic fusagene vectors, Tenth International Conference on Cancer Gene Therapy, July 2001, London, UK.

Hejazi M S, Gaken J, Farzaneh F. Development of a new strategy for cytoplasmic fusagene vectors for expression of multiple genes from a single expression cassette, Sixth Iranian Conference of Biochemistry, October 2001, Tehran, Iran.

Zanguei J, Hejazi M S, Adrangi M. Formulation of parenteral suspension of medroxyprogestrone acetate, The fourth Congress of pharmaceutical sciences, September 1994, Tehran, Iran.

Rostami A, Hejazi M S. A simple spectrophotometric method for analysis of drug mixtures, The Second Iranian Congress of Pharmaceutical Sciences, October 1992, Mashhad, Iran.

Hejazi M S, Jalali M. Application of flocculation processes in formulation of suspensions. The Second Iranian Congress of Pharmaceutical Sciences, October 1992, Mashhad, Iran.

Hejazi M S, Jalali M. Effects of smoking on drug disposition, The Second Iranian Congress of Poisoning, October 1991, Tabriz, Iran

 

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